Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR)

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Abstract

Fourier transformed mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls (Acebes et al., Front Plant Sci 5:303, 2014; Badhan et al., Biotechnol Biofuels 7:1–15, 2014; Badhan et al., BioMed Res Int 2015: 562952, 2015; Roach et al., Plant Physiol 156:1351–1363, 2011). The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here, we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall. This method has been successfully used to study complex enzyme saccharification processes like rumen digestion to identify recalcitrant moieties in low-quality forage which resist rumen digestion (Badhan et al., BioMed Res Int 2015: 562952, 2015), as well as to characterize cell wall mutant lines or transgenic lines expressing exogenous hydrolases (Badhan et al., Biotechnol Biofuels 7:1–15, 2014; Roach et al., Plant Physiol 156:1351–1363, 2011). The FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low cost and nondestructive manner.

Key words

Fourier transformed infrared spectroscopy Plant cell wall Carbohydrate Cellulose Xylan Lignin 

Notes

Acknowledgment

The authors wish to acknowledge the support of Cellulosic Biofuel Network of Agriculture and Agri-Food Canada, Genome Canada, Genome Alberta, and Genome Quebec for their generous support of their research program.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Lethbridge Research and Development Centre, Agriculture and Agri-Food CanadaLethbridgeCanada

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