CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry

  • Louise Badruna
  • Vincent Burlat
  • Cédric Y. Montanier
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Abstract

Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated polysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction.

Key words

Wheat straw section Immunocytochemistry Enzymatic hydrolysis Fluorescence Microscopy 

Notes

Acknowledgments

We acknowledge Alain Jauneau and Aurelie Le Ru for technical assistance on Nanozoomer RS provided by the TRI-genotoul facility (http://trigenotoul.com/).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Louise Badruna
    • 1
  • Vincent Burlat
    • 2
  • Cédric Y. Montanier
    • 1
  1. 1.LISBPUniversité de Toulouse, CNRS, INRA, INSAToulouseFrance
  2. 2.Laboratoire de Recherche en Sciences VégétalesCastanet-TolosanFrance

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