Bioinspired Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP

  • Gabriel Paës
Part of the Methods in Molecular Biology book series (MIMB, volume 1588)


The interactions of proteins involved in plant cell wall hydrolysis, such as enzymes and CBMs, significantly determine their role and efficiency. In order to go beyond the characterization of interactions with simple ligands, bioinspired assemblies combined with the measurement of diffusion and interaction by FRAP offer a relevant alternative for highlighting the importance of different parameters related to the protein affinity and to the assembly.

Key words

Bioinspired Diffusion Interaction Ligand Protein Enzyme CBM FRAP 



This work was supported by INRA and the French National Research Agency (LIGNOPROG project ANR-14-CE05-0026).


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.FARE laboratory, INRAUniversity of Reims Champagne-ArdenneReimsFrance

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