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Techniques to Investigate Bioenergetics of Mitochondria

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Techniques to Investigate Mitochondrial Function in Neurons

Part of the book series: Neuromethods ((NM,volume 123))

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Abstract

In this chapter we review basic mitochondrial physiology as related to respiration, membrane potential, and ATP production and the relationship to ROS and calcium . Methods for measuring various functional parameters are described. Techniques differ according to whether one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per se, mitochondrial function clearly is dependent on a multitude of cellular and whole body factors.

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Author information

Authors and Affiliations

  1. Division of Endocrinology and Metabolism, Department of Internal Medicine, The University of Iowa Hospitals and Clinics, 422GH, 200 Hawkins Drive, Iowa City, IA, 52242, USA

    William I. Sivitz

  2. Iowa City VAMC, Iowa City, IA, USA

    William I. Sivitz

Authors
  1. William I. Sivitz
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Correspondence to William I. Sivitz .

Editor information

Editors and Affiliations

  1. Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA

    Stefan Strack

  2. Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA

    Yuriy M. Usachev

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Sivitz, W.I. (2017). Techniques to Investigate Bioenergetics of Mitochondria. In: Strack, S., Usachev, Y. (eds) Techniques to Investigate Mitochondrial Function in Neurons. Neuromethods, vol 123. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6890-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6890-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6888-6

  • Online ISBN: 978-1-4939-6890-9

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