High-Throughput Production of Proteins in E. coli for Structural Studies
We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target proteins. It includes a one-step purification for the purposes of functional assays and a two-step protocol for crystallographic studies, with the option of on-column tag removal.
Key wordsProtein purification Parallel purification High-throughput ÄKTA-Xpress E. coli expression Expression triage
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