A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis

  • Trang Phan
  • Phuong Huynh
  • Tuom Truong
  • Hoang Nguyen
Part of the Methods in Molecular Biology book series (MIMB, volume 1586)


Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and purified using this host. In this chapter, we introduce a generic protocol to express a recombinant protein in B. subtilis. It includes protocols for (1) using our typical expression vector (plasmid pHT254) to introduce a target gene, (2) transformation of the target vector into B. subtilis, and (3) evaluation of the actual expression of a recombinant protein.

Key words

Bacillus subtilis pHT01 vector Pgrac promoter pHT254 Pgrac100 promoter 



This research was funded by the Vietnam National Foundation for Science and Technology Development (Nafosted) under grant number 106-NN.02-2015.24. The research was also supported by TWAS under research grants 14-201 RG/BIO/AS_G.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Trang Phan
    • 1
  • Phuong Huynh
    • 1
  • Tuom Truong
    • 1
  • Hoang Nguyen
    • 1
  1. 1.VNUHCM-University of ScienceHochiminh CityVietnam

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