A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis
Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and purified using this host. In this chapter, we introduce a generic protocol to express a recombinant protein in B. subtilis. It includes protocols for (1) using our typical expression vector (plasmid pHT254) to introduce a target gene, (2) transformation of the target vector into B. subtilis, and (3) evaluation of the actual expression of a recombinant protein.
Key wordsBacillus subtilis pHT01 vector Pgrac promoter pHT254 Pgrac100 promoter
This research was funded by the Vietnam National Foundation for Science and Technology Development (Nafosted) under grant number 106-NN.02-2015.24. The research was also supported by TWAS under research grants 14-201 RG/BIO/AS_G.