Expression of Prokaryotic Integral Membrane Proteins in E. coli

  • James D. Love
Part of the Methods in Molecular Biology book series (MIMB, volume 1586)


Production of prokaryotic membrane proteins for structural and functional studies in E. coli can be parallelized and miniaturized. All stages from cloning, expression, purification to detergent selection can be investigated using high-throughput techniques to rapidly and economically find tractable targets.

Key words

High throughput Membrane proteins Detergent assay Multiwell 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of BiochemistryAlbert Einstein College of Medicine at Yeshiva UniversityBronxUSA
  2. 2.ATUMNewarkUSA

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