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Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications

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Human T-Lymphotropic Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1582))

Abstract

Post-translational modifications (PTMs) are chemical alterations to individual amino acids that alter a protein’s conformation, stability, and/or function. Several pathogenic viruses have been shown to encode proteins with PTMs, including human T-cell leukemia virus type 1 (HTLV-1) Tax and Rex regulatory proteins. HTLV-1 basic leucine zipper protein (HBZ) was hypothesized to feature PTMs due to its functional activities and interactions with cellular transcription factors and acetyltransferases. Here, we describe the approach used to identify, via mass spectrometry, the PTMs of HBZ. In addition, we describe methods to determine the functional relevance of the identified PTMs.

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Author information

Authors and Affiliations

  1. Center for Retrovirus Research, The Ohio State University, Columbus, OH, 43210, USA

    Jacob Al-Saleem, Mamuka Kvaratskhelia & Patrick L. Green

  2. Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, 43210, USA

    Jacob Al-Saleem & Patrick L. Green

  3. College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA

    Mamuka Kvaratskhelia

  4. Compreshensive Cancer Center and Solove Research Institute, The Ohio State University, Columbus, OH, 43210, USA

    Patrick L. Green

  5. Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, 127N Veterinary Medicine Academic Building, 1900 Coffey Rd, Columbus, OH, 43210, USA

    Patrick L. Green

Authors
  1. Jacob Al-Saleem
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  2. Mamuka Kvaratskhelia
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  3. Patrick L. Green
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Corresponding author

Correspondence to Patrick L. Green .

Editor information

Editors and Affiliations

  1. University of Milano, Milano, Italy

    Claudio Casoli

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Al-Saleem, J., Kvaratskhelia, M., Green, P.L. (2017). Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications. In: Casoli, C. (eds) Human T-Lymphotropic Viruses. Methods in Molecular Biology, vol 1582. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6872-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6872-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6870-1

  • Online ISBN: 978-1-4939-6872-5

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