Abstract
A MAP kinase pathway below a chitin receptor in the moss Physcomitrella patens induces immune responses including rapid growth inhibition, a novel fluorescence burst, and cell wall depositions. The molecular mechanisms producing these three responses are currently unknown but warrant further investigation in this simple model system. Here we describe qualitative, time-lapse, and quantitative assays to monitor and measure these responses.
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Acknowledgments
This work was supported by grants from the Danish Basic Research Foundation (Center for Comparative Genomics) and the Danish Research Council for Nature and the Universe (Comparative and functional genomics of plant innate immunity, 1323-00267A) to J.M.
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Bressendorff, S., Rasmussen, M.W., Petersen, M., Mundy, J. (2017). Chitin-Induced Responses in the Moss Physcomitrella patens . In: Shan, L., He, P. (eds) Plant Pattern Recognition Receptors. Methods in Molecular Biology, vol 1578. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6859-6_27
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DOI: https://doi.org/10.1007/978-1-4939-6859-6_27
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