Chitin-Induced Responses in the Moss Physcomitrella patens

  • Simon Bressendorff
  • Magnus Wohlfahrt Rasmussen
  • Morten Petersen
  • John MundyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)


A MAP kinase pathway below a chitin receptor in the moss Physcomitrella patens induces immune responses including rapid growth inhibition, a novel fluorescence burst, and cell wall depositions. The molecular mechanisms producing these three responses are currently unknown but warrant further investigation in this simple model system. Here we describe qualitative, time-lapse, and quantitative assays to monitor and measure these responses.

Key words

Bryophyte Plant innate immunity 



This work was supported by grants from the Danish Basic Research Foundation (Center for Comparative Genomics) and the Danish Research Council for Nature and the Universe (Comparative and functional genomics of plant innate immunity, 1323-00267A) to J.M.


  1. 1.
    Clarke JT, Warnock RCM, Donoghue PCJ (2011) Establishing a time-scale for plant evolution. New Phytol 192:266–301CrossRefPubMedGoogle Scholar
  2. 2.
    Schaefer D, Zryd J-P, Knight CD, Cove DJ (1991) Stable transformation of the moss Physcomitrella patens. Mol Gen Genet 226:418–424CrossRefPubMedGoogle Scholar
  3. 3.
    Mittag J, Šola I, Rusak G, Ludwig-Müller J (2015) Physcomitrella patens auxin conjugate synthetase (GH3) double knockout mutants are more resistant to Pythium infection than wild type. J Plant Physiol 183:75–83CrossRefPubMedGoogle Scholar
  4. 4.
    Oliver J, Castro A, Gaggero C, Cascón T, Schmelz E, Castresana C, Ponce de León I (2009) Pythium infection activates conserved plant defense responses in mosses. Planta 230:569–579CrossRefPubMedGoogle Scholar
  5. 5.
    Ponce De León I, Oliver J, Castro A, Gaggero C, Bentancor M, Vidal S (2007) Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patens. BMC Plant Biol 7:52CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Ponce De León I, Schmelz EA, Gaggero C, Castro A, Álvarez A, Montesano M (2012) Physcomitrella patens activates reinforcement of the cell wall, programmed cell death and accumulation of evolutionary conserved defence signals, such as salicylic acid and 12-oxo-phytodienoic acid, but not jasmonic acid, upon Botrytis cinerea infection. Mol Plant Pathol 13:960–974CrossRefPubMedGoogle Scholar
  7. 7.
    Reboledo G, del Campo R, Alvarez A, Montesano M, Mara H, Ponce de León I (2015) Physcomitrella patens activates defense responses against the pathogen Colletotrichum gloeosporioides. Int J Mol Sci 16:22280–22298CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Ponce de León I, Montesano M (2013) Activation of defense mechanisms against pathogens in mosses and flowering plants. Int J Mol Sci 14:3178–3200CrossRefPubMedGoogle Scholar
  9. 9.
    Lehtonen MT, Akita M, Frank W, Reski R, Valkonen JPT (2012) Involvement of a class III peroxidase and the mitochondrial protein TSPO in oxidative burst upon treatment of moss plants with a fungal elicitor. Mol Plant Microbe Interact 25:363–371CrossRefPubMedGoogle Scholar
  10. 10.
    Lehtonen MT, Takikawa Y, Rönnholm G, Akita M, Kalkkinen N, Ahola-Iivarinen E, Somervuo P, Varjosalo M, Valkonen JPT (2014) Protein secretome of moss plants (Physcomitrella patens) with emphasis on changes induced by a fungal elicitor. J Proteome Res 13(2):447–459CrossRefPubMedGoogle Scholar
  11. 11.
    Bressendorff S, Azevedo R, Kenchappa CS, Ponce de León I, Rasmussen MW, Erbs G, Newmann M, Petersen M, Mundy J (2016) An innate immunity pathway in the moss Physcomitrella patens. Plant Cell 28:1328–1342CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Gómez-Gómez L, Felix G, Boller T (1999) A single locus determines sensitivity to bacterial flagellin in Arabidopsis thaliana. Plant J 18:277–284CrossRefPubMedGoogle Scholar
  13. 13.
    Li J, Zhao-Hui C, Batoux M, Nekrasov V, Roux M, Chinchilla D, Zipfel C, Jones JDG (2009) Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR. Proc Natl Acad Sci U S A 106:15973–15978CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Beasley CA, Birnbaum EH, Dugger WM, Ting IP (1974) A quantitative procedure for estimating cotton fiber growth. Biotech Histochem 49:85–92Google Scholar
  15. 15.
    Ashton NW, Cove DJ (1977) The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss Physcomitrella patens. Mol Gen Genet 154:87–95CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Simon Bressendorff
    • 1
  • Magnus Wohlfahrt Rasmussen
    • 1
  • Morten Petersen
    • 1
  • John Mundy
    • 1
    Email author
  1. 1.Department of BiologyUniversity of CopenhagenCopenhagenDenmark

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