Utilization of Selenocysteine for Site-Specific Antibody Conjugation

Part of the Methods in Molecular Biology book series (MIMB, volume 1575)


Site-specific conjugation methods are becoming increasingly important in building next-generation antibody-drug conjugates. We have developed a site-specific conjugation technology based on monoclonal antibodies with engineered selenocysteine (Sec) residues, named selenomabs. Here, we provide protocols for the engineering, expression, and purification of selenomabs in single-chain variable fragment (scFv)-Fc format. Methods for selective conjugation of selenomabs to selenol-reactive compounds and analytical characterization of selenomab conjugates are also included.

Key words

Antibody-drug conjugates Site-specific conjugation Selenomab Selenocysteine 


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Cancer BiologyThe Scripps Research InstituteJupiterUSA
  2. 2.Department of Molecular TherapeuticsThe Scripps Research InstituteJupiterUSA

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