Whole Cell Panning with Phage Display

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1575)

Abstract

Phage display has emerged as one of the leading technologies for the selection of highly specific monoclonal antibodies, offering a number of advantages over traditional methods of antibody generation. While there are various possibilities to conduct phage display (e.g., solution panning, solid-phase panning), whole cell panning is an elegant way to present membrane embedded target antigens in their natural environment and conformation to antibody-bearing phages. Here, a whole cell panning procedure using a Fab-based antibody library including primary cell based screening for selectivity is described.

Key words

Antibody fragment Phage display Antibody library Whole cell panning (WCP) 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.MorphoSys AGPlaneggGermany

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