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Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection

  • Francesco Canfarotta
  • Katarzyna Smolinska-Kempisty
  • Sergey Piletsky
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1575)

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a widely employed analytical test used to quantify a given molecule. It relies on the use of specific antibodies, linked to an enzyme, to target the desired molecule. The reaction between the enzyme and its substrate gives rise to the analytical signal that can be quantified. Thanks to their robustness and low cost, molecularly imprinted polymer nanoparticles (nanoMIPs) are a viable alternative to antibodies. Herein, we describe the synthesis of nanoMIPs imprinted for vancomycin and their subsequent application in an ELISA-like format for direct replacement of antibodies.

Key words

Molecularly imprinted nanoparticles Diagnostics Assay Analyte quantification 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Francesco Canfarotta
    • 1
  • Katarzyna Smolinska-Kempisty
    • 2
  • Sergey Piletsky
    • 2
  1. 1.MIP Diagnostics Ltd.University of LeicesterLeicesterUK
  2. 2.Department of ChemistryUniversity of LeicesterLeicesterUK

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