H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers

  • Chak Sangma
  • Peter A. Lieberzeit
  • Wannisa Sukjee
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1575)

Abstract

Normally, antibodies against influenza A have been prepared from viable virus or an engineered strain in certain hosts or cultured media. Two factors concerning antibody production are obvious. The obtaining antibody that is a kind of biomolecule has to be handled carefully, e.g., to be kept in a refrigerator. Furthermore, when the virus strain is highly pathogenic, such as H5N1, antibody production has to be done carefully in a high-level biosafety lab. Here, we show how to produce an antibody against H5N1 from a polymeric material using inactivated virus which can be conducted in a low-level biosafety lab. The process is based on imprinting the whole virus on a polymer surface to form molecularly imprinted polymers (MIPs). The MIPs show some properties of H5N1 antibody as they recognize H5N1 and have some important antibody activity. The H5N1 MIPs are not to be considered biomaterial, so they can be stored at room temperature and thus do not need any special care.

Key words

Plastic antibody H5N1 influenza virus Molecularly imprinted polymers Surface imprinting H5N1 MIPs Synthetic H5N1 antibody 

Notes

Acknowledgments

This work has been supported by Royal Golden Jubilee Ph.D. Program (RGJ), Thailand Research Fund (TRF), Kasetsart University Research and Development Institute (KURDI), the National Research University Project of Thailand (NRU), and ASEAN-European Academic University Network (ASEA-UNINET).

References

  1. 1.
    Edwin DK (2006) Influenza pandemics of the 20th century. Emerg Infect Dis 12(1):9. doi: 10.3201/eid1201.051254 CrossRefGoogle Scholar
  2. 2.
    Beveridge WI (1991) The chronicle of influenza epidemics. Hist Philos Life Sci 13(2):223–234PubMedGoogle Scholar
  3. 3.
    Jeffery T, David MM (2006) 1918 Influenza: the mother of all pandemics. Emerg Infect Dis 12(1):15. doi: 10.3201/eid1201.050979 CrossRefGoogle Scholar
  4. 4.
    Taubenberger JK (2006) The origin and virulence of the 1918 “Spanish” influenza virus. Proc Am Philos Soc 150(1):86–112PubMedPubMedCentralGoogle Scholar
  5. 5.
    Watanabe T, Kawaoka Y (2011) Pathogenesis of the 1918 pandemic influenza virus. PLoS Pathog 7(1):e1001218. doi: 10.1371/journal.ppat.1001218 CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Kim H-R, Lee Y-J, Park C-K, Oem J-K, Lee OS, Kang H-M, Choi J-G, Bae Y-C (2012) Highly pathogenic avian influenza (H5N1) outbreaks in wild birds and poultry, South Korea. Emerg Infect Dis 18(3):480–483. doi: 10.3201/eid1803.111490 CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Eagles D, Siregar ES, Dung DH, Weaver J, Wong F, Daniels P (2009) H5N1 highly pathogenic avian influenza in Southeast Asia. Rev Sci Tech 28(1):341–348PubMedGoogle Scholar
  8. 8.
    Gutiérrez RA, Naughtin MJ, Horm SV, San S, Buchy P (2009) A(H5N1) virus evolution in South East Asia. Viruses 1(3):335–361. doi: 10.3390/v1030335 CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Wang H, Feng Z, Shu Y, Yu H, Zhou L, Zu R, Huai Y, Dong J, Bao C, Wen L, Wang H, Yang P, Zhao W, Dong L, Zhou M, Liao Q, Yang H, Wang M, Lu X, Shi Z, Wang W, Gu L, Zhu F, Li Q, Yin W, Yang W, Li D, Uyeki TM, Wang Y (2008) Probable limited person-to-person transmission of highly pathogenic avian influenza A (H5N1) virus in China. Lancet 371(9622):1427–1434. doi: 10.1016/S0140-6736(08)60493-6 CrossRefPubMedGoogle Scholar
  10. 10.
    Ungchusak K, Auewarakul P, Dowell SF, Kitphati R, Auwanit W, Puthavathana P, Uiprasertkul M, Boonnak K, Pittayawonganon C, Cox NJ, Zaki SR, Thawatsupha P, Chittaganpitch M, Khontong R, Simmerman JM, Chunsutthiwat S (2005) Probable person-to-person transmission of avian influenza A (H5N1). N Engl J Med 352(4):333–340. doi: 10.1056/NEJMoa044021 CrossRefPubMedGoogle Scholar
  11. 11.
    Gambotto A, Barratt-Boyes SM, de Jong MD, Neumann G, Kawaoka Y (2008) Human infection with highly pathogenic H5N1 influenza virus. Lancet 371(9622):1464–1475. doi: 10.1016/S0140-6736(08)60627-3 CrossRefPubMedGoogle Scholar
  12. 12.
    Baz M, Luke CJ, Cheng X, Jin H, Subbarao K (2013) H5N1 vaccines in humans. Virus Res 178(1):78–98. doi: 10.1016/j.virusres.2013.05.006 CrossRefPubMedGoogle Scholar
  13. 13.
    Kang S-M, Yoo D-G, Lipatov AS, Song J-M, Davis CT, Quan F-S, Chen L-M, Donis RO, Compans RW (2009) Induction of long-term protective immune responses by influenza H5N1 virus-like particles. PLoS One 4(3):e4667. doi: 10.1371/journal.pone.0004667 CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Wangchareansak T, Thitithanyanont A, Chuakheaw D, Gleeson MP, Lieberzeit PA, Sangma C (2013) Influenza A virus molecularly imprinted polymers and their application in virus sub-type classification. J Mater Chem B 1(16):2190–2197. doi: 10.1039/C3TB00027C CrossRefGoogle Scholar
  15. 15.
    Thitithanyanont A, Engering A, Ekchariyawat P, Wiboon-ut S, Limsalakpetch A, Yongvanitchit K, Kum-Arb U, Kanchongkittiphon W, Utaisincharoen P, Sirisinha S, Puthavathana P, Fukuda MM, Pichyangkul S (2007) High susceptibility of human dendritic cells to avian influenza H5N1 virus infection and protection by IFN-α and TLR ligands. J Immunol 179(8):5220–5227. doi: 10.4049/jimmunol.179.8.5220 CrossRefPubMedGoogle Scholar
  16. 16.
    Eisfeld AJ, Neumann G, Kawaoka Y (2014) Influenza A virus isolation, culture and identification. Nat Protoc 9(11):2663–2681. doi: 10.1038/nprot.2014.180 CrossRefPubMedGoogle Scholar
  17. 17.
    Wangchareansak T, Thitithanyanont A, Chuakheaw D, Gleeson MP, Lieberzeit PA, Sangma C (2014) A novel approach to identify molecular binding to the influenza virus H5N1: screening using molecularly imprinted polymers (MIPs). Med Chem Commun 5(5):617–621. doi: 10.1039/C3MD00272A CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Chak Sangma
    • 1
  • Peter A. Lieberzeit
    • 2
  • Wannisa Sukjee
    • 3
  1. 1.Faculty of Science, Department of Chemistry, Center for Advanced Studies in Nanotechnology and Its Applications in Chemical, Food and Agricultural IndustriesKasetsart UniversityBangkokThailand
  2. 2.Department of Analytical ChemistryUniversity of ViennaViennaAustria
  3. 3.Faculty of Science, Department of ChemistryKasetsart UniversityBangkokThailand

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