Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization
Molecularly imprinted polymers (MIPs) gained an expansively growing interest in the past few decades. After an initial, explorative period of preparing MIPs exclusively with bulk polymerization, new polymer synthesis routes have been adapted to overcome the drawbacks of the traditional method. Among these the most appealing is precipitation polymerization that results in nano- and microspheres with narrow size distribution and makes the production of MIPs more straightforward. Here, we describe a precipitation polymerization protocol for a common small molecule template, propranolol that is carried out in the conventional way, in dilute monomer solution. Moreover, a modified precipitation polymerization protocol from concentrated monomer solution is presented for a diclofenac imprinted polymer which makes the synthesis even more versatile and circumvents the disadvantages of the dilute solution conditions.
Key wordsMolecularly imprinted polymer Polymer microsphere Precipitation polymerization Monodisperse Modified precipitation polymerization Propranolol Diclofenac
The authors gratefully acknowledge the financial support of the Hungarian Scientific Fund (Grant K104724).
- 1.van Herk AM, Monteiro M (2002) Heterogeneous systems. In: Matyjaszewski K, Davis TP (eds) Handbook of radical polymerization. John Wiley and Sons, Inc., HobokenGoogle Scholar
- 5.Perez N, Whitcombe MJ, Vulfson EN (2000) Molecularly imprinted nanoparticles prepared by core-shell emulsion polymerization. J Appl Polym Sci 77(8):1851–1859. doi: 10.1002/1097-4628(20000822)77:81851::aid-app23>3.0.co;2-j
- 6.Vaihinger D, Landfester K, Krauter I, Brunner H, Tovar GEM (2002) Molecularly imprinted polymer nanospheres as synthetic affinity receptors obtained by miniemulsion polymerisation. Macromol Chem Phys 203(13):1965–1973. doi: 10.1002/1521-3935(200209)203:13<1965::aid-macp1965>3.0.co;2-c CrossRefGoogle Scholar
- 16.Benito-Pena E, Navarro-Villoslada F, Carrasco S, Jockusch S, Ottaviani MF, Moreno-Bondi MC (2015) Experimental mixture design as a tool for the synthesis of antimicrobial selective molecularly imprinted monodisperse microbeads. ACS Appl Mater Interfaces 7(20):10966–10976. doi: 10.1021/acsami.5b02238 CrossRefPubMedGoogle Scholar
- 20.Yoshimatsu K, Reimhult K, Krozer A, Mosbach K, Sode K, Ye L (2007) Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: The control of particle size suitable for different analytical applications. Anal Chim Acta 584(1):112–121CrossRefPubMedGoogle Scholar
- 22.Li WH, Stover HDH (1998) Porous monodisperse poly(divinylbenzene) microspheres by precipitation polymerization. J Polym Sci Part A Polym Chem 36(10):1543–1551. doi: 10.1002/(sici)1099-0518(19980730)36:10<1543::aid-pola7>3.0.co;2-r CrossRefGoogle Scholar
- 25.Moreno-Bondi MC, Urraca JL, Carrasco S, Navarro-Villoslada F (2013) Preparation of molecularly imprinted polymers. In: Alvarez-Lorenzo C, Concheiro A (eds) Handbook of molecularly imprinted polymers. Smithers Rapra, Shawbury, Shrewsbury, pp 23–86Google Scholar