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Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer

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Synthetic Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1575))

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Abstract

Natural antibodies are widely used for their unprecedented reproducibility and the remarkable selectivity for a wide range of analytes. However, biodegradability and the need to work in biocompatible environments limit their applications. Molecularly imprinted polymers are a robust alternative. While molecularly imprinted polymers have shown remarkable selectivities for small molecules, large structures as proteins, viruses or entire cells are still problematic and flexible structures are virtually impossible to imprint. We have developed a method to form a polymeric copy of the antibodies instead. This book chapter aims to summarize the progress with this technique. To make it easier for other scientists to use this methods I critically discuss advantages and drawbacks of the method compared to alternative techniques. The discussion should help to identify for which applications this technique would be valuable. Finally, I provide a practical guide to use this new method. I highlight potential problems and give hints for possible improvements or adaptations for different applications.

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Author information

Authors and Affiliations

  1. University Medical Center Groningen, Groningen University, Antonius Deusinglaan 1, 9713 AW, Groningen, The Netherlands

    Romana Schirhagl

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  1. Romana Schirhagl
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Correspondence to Romana Schirhagl .

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Editors and Affiliations

  1. MorphoSys AG, Discovery Alliances & Technologies, Planegg, Germany

    Thomas Tiller

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Schirhagl, R. (2017). Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer. In: Tiller, T. (eds) Synthetic Antibodies. Methods in Molecular Biology, vol 1575. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6857-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6857-2_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6855-8

  • Online ISBN: 978-1-4939-6857-2

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