A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells
There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.
Key wordsProximity-based assay Ligand Membrane protein Interaction Reporter cell Lentivirus
This work was supported by JPB foundation and Zebra Biologics.
- 7.Kirby AJ (1981) Effective Molarities for Intramolecular Reactions. Adv Phys Org Chem 17:183–278Google Scholar