A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1575)

Abstract

There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.

Key words

Proximity-based assay Ligand Membrane protein Interaction Reporter cell Lentivirus 

Notes

Acknowledgment

This work was supported by JPB foundation and Zebra Biologics.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Cell and Molecular BiologyThe Scripps Research InstituteLa JollaUSA

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