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Protein Terminal Profiling pp 9–15Cite as

DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity

Part of the Methods in Molecular Biology book series (MIMB,volume 1574)

Abstract

We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.

Key words

  • N-terminal acetyltransferase (NAT)
  • Acetyltransferase
  • Enzyme activity
  • Enzyme assay
  • 5,5′-dithiobis-(2-nitrobenzoic acid)
  • Thiol quantification
  • Thiol detection
  • Ac-CoA

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Acknowledgments

These studies were supported by grants from the Norwegian Cancer Society (to T.A.), The Bergen Research Foundation BFS (to T.A.), the Research Council of Norway (grant 230865 to T.A), and the Western Norway Regional Health Authority (to T.A.).

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Authors and Affiliations

  1. Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway

    Håvard Foyn & Thomas Arnesen

  2. Department of Biochemistry and Molecular Pharmacology, UMASS Medical School, 365 Plantation Street, Worcester, MA, 01605, USA

    Paul R. Thompson

  3. Department of Surgery, Haukeland University Hospital, N-5021, Bergen, Norway

    Thomas Arnesen

Authors
  1. Håvard Foyn
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  2. Paul R. Thompson
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  3. Thomas Arnesen
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Corresponding author

Correspondence to Thomas Arnesen .

Editor information

Editors and Affiliations

  1. Institute of Molecular Medicine and Cell Research, University Freiburg, Freiburg, Germany

    Oliver Schilling

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Foyn, H., Thompson, P.R., Arnesen, T. (2017). DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity. In: Schilling, O. (eds) Protein Terminal Profiling. Methods in Molecular Biology, vol 1574. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6850-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6850-3_2

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  • Publisher Name: Humana Press, New York, NY

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  • Online ISBN: 978-1-4939-6850-3

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