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Biosensors and Biodetection pp 327–341Cite as

Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms Through Chemical Modification and Incorporation of Nanoparticles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1571))

Abstract

This chapter describes two different methodologies used to improve the analytical performance of colorimetric paper-based biosensors. Microfluidic paper-based analytical devices (μPADs) have been produced by a stamping process and CO2 laser ablation and modified, respectively, through an oxidation step and incorporation of silica nanoparticles on the paper structure. Both methods are employed in order to overcome the largest problem associated with colorimetric detection, the heterogeneity of the color distribution in the detection zones. The modification steps are necessary to improve the interaction between the paper surface and the selected enzymes. The enhanced performance has ensured reliability for quantitative analysis of clinically relevant compounds.

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Author information

Authors and Affiliations

  1. Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil

    Ellen Flávia Moreira Gabriel, Paulo T. Garcia, Thiago M. G. Cardoso & Wendell K. T. Coltro

  2. Department of Chemistry, Clemson University, Clemson, SC 29634, TX, 78249, USA

    Elizabeth Evans & Carlos D. Garcia

  3. Instituto Nacional de Ciência e Tecnologia de Bioanalítica (INCTBio), Campinas, SP, Brazil

    Wendell K. T. Coltro

Authors
  1. Ellen Flávia Moreira Gabriel
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  2. Paulo T. Garcia
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  3. Elizabeth Evans
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  4. Thiago M. G. Cardoso
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  5. Carlos D. Garcia
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  6. Wendell K. T. Coltro
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Corresponding author

Correspondence to Wendell K. T. Coltro .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

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Gabriel, E.F.M., Garcia, P.T., Evans, E., Cardoso, T.M.G., Garcia, C.D., Coltro , W.K.T. (2017). Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms Through Chemical Modification and Incorporation of Nanoparticles. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6848-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6846-6

  • Online ISBN: 978-1-4939-6848-0

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