Abstract
Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance. The advantages of direct optical detection depend on a sophisticated surface chemistry resulting negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gauglitz G (2010) Direct optical detection in bioanalysis: an update. Anal Bioanal Chem 398(6):2363–2372
Homola J, Yee SS, Gauglitz G (1999) Surface plasmon resonance sensors: review. Sens Actuators B 54:3–15
Schmitt HM, Brecht A, Piehler J, Gauglitz G (1997) An integrated system for optical biomolecular interaction analysis. Biosens Bioelectron 12:219–233
Brecht A, Gauglitz G, Nahm W (1992) Interferometric measurements used in chemical and biochemical sensors. Analysis 20:135–140
Brecht A, Gauglitz G, Kraus G, Nahm W (1993) Chemical and biochemical sensors based on interferometry at thin layers. Sens Actuators 11B:21–27
Proell F, Moehrle B, Kumpf M, Gauglitz G (2005) Label-free characterisation of oligonucleotide hybridisation using reflectometric interference spectroscopy. Anal Bioanal Chem 382(2):1889–1894
Fechner P, Pröll F, Albrecht C, Gauglitz G (2011) Kinetic analysis of the estrogen receptor alpha using RIfS. Anal Bioanal Chem 400(3):729–735
Pröll F, Fechner P, Proll G (2009) Direct optical detection in fragment-based screening. Anal Bioanal Chem 393(6–7):1557–1562
Kolarov F, Niedergall K, Bach M, Tovar GEM, Gauglitz G (2012) Optical sensors with molecularly imprinted nanospheres: a promising approach for robust and label free detection of small molecules. Anal Bioanal Chem 402(10):3245–3252
Ewald M, Le Blanc AF, Gauglitz G, Proll G (2013) A robust sensor platform for label-free detection of anti-Salmonella antibodies using undiluted animal sera. Anal Bioanal Chem 405(20):6461–6469. doi:10.1007/s00216-013-7040-9
Krieg AK, Gauglitz G (2014) An optical sensor for the detection of human pancreatic lipase. Sens Actuators B 203:663–669. doi:10.1016/j.snb.2014.07.036
Ewald M, Fechner P, Gauglitz G (2015) A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing. Anal Bioanal Chem 407(14):4005–4013. doi:10.1007/s00216-015-8562-0
Rau S, Hilbig U, Gauglitz G (2014) Label-free optical biosensor for detection and quantification of the non-steroidal anti-inflammatory drug diclofenac in milk without any sample pretreatment. Anal Bioanal Chem 406(14):3377–3386. doi:10.1007/s00216-014-7755-2
Frank R, Möhrle B, Fröhlich D, Gauglitz G (2005) A lable-free detection method of biochemical interactions with low-cost plastic and other transperent transducers. Proc SPIE Int Soc Opt Eng 5826:551–560
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Proll, G., Markovic, G., Fechner, P., Proell, F., Gauglitz, G. (2017). Reflectometric Interference Spectroscopy. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6848-0_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6846-6
Online ISBN: 978-1-4939-6848-0
eBook Packages: Springer Protocols