Abstract
In this chapter, we describe the preparation of glucose-responsive vesicles (GRVs) and the fabrication of GRV-loaded microneedle-array patches for insulin delivery. The GRVs were formed of hypoxia-sensitive hyaluronic acid (HS-HA), the synthesis of which is presented in detail. We also describe the procedure to evaluate the in vivo efficacy of this smart patch in a mouse model of chemically induced type 1 diabetes through transcutaneous administration.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Mo R et al (2014) Emerging micro-and nanotechnology based synthetic approaches for insulin delivery. Chem Soc Rev 43(10):3595–3629
Veiseh O et al (2015) Managing diabetes with nanomedicine: challenges and opportunities. Nat Rev Drug Discov 14(1):45–57
Owens DR, Zinman B, Bolli GB (2001) Insulins today and beyond. Lancet 358(9283):739–746
Bratlie KM et al (2012) Materials for diabetes therapeutics. Adv Healthc Mater 1(3):267–284
Ohkubo Y et al (1995) Intensive insulin therapy prevents the progression of diabetic microvascular complications in Japanese patients with non-insulin-dependent diabetes mellitus: a randomized prospective 6-year study. Diabetes Res Clin Pract 28(2):103–117
Ravaine V, Ancla C, Catargi B (2008) Chemically controlled closed-loop insulin delivery. J Control Release 132(1):2–11
Gu Z et al (2013) Injectable nano-network for glucose-mediated insulin delivery. ACS Nano 7(5):4194–4201
Gu Z et al (2013) Glucose-responsive microgels integrated with enzyme nanocapsules for closed-loop insulin delivery. ACS Nano 7(8):6758–6766
Tai W et al (2014) Bio-inspired synthetic nanovesicles for glucose-responsive release of insulin. Biomacromolecules 15(10):3495–3502
Chou DH-C et al (2015) Glucose-responsive insulin activity by covalent modification with aliphatic phenylboronic acid conjugates. Proc Natl Acad Sci 112(8):2401–2406
Kim SW et al (1990) Self-regulated glycosylated insulin delivery. J Control Release 11(1):193–201
Yu J et al (2015) Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery. Proc Natl Acad Sci 112(27):8260–8265
Nunn A, Linder K, Strauss HW (1995) Nitroimidazoles and imaging hypoxia. Eur J Nucl Med 22(3):265–280
Krohn KA, Link JM, Mason RP (2008) Molecular imaging of hypoxia. J Nucl Med 49(Suppl 2):129S–148S
Prausnitz MR (2004) Microneedles for transdermal drug delivery. Adv Drug Deliv Rev 56(5):581–587
Yang SY et al (2013) A bio-inspired swellable microneedle adhesive for mechanical interlocking with tissue. Nat Commun 4:1702
Heo YJ et al (2011) Long-term in vivo glucose monitoring using fluorescent hydrogel fibers. Proc Natl Acad Sci 108(33):13399–13403
Acknowledgment
This work was supported by the grants from the American Diabetes Association (ADA) to Z.G. (1-14-JF-29 and 1-15-ACE-21) and a grant from NC TraCS, NIH’s Clinical and Translational Science Awards (CTSA, NIH grant 1UL1TR001111) at UNC-CH.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Yu, J., Zhang, Y., Gu, Z. (2017). Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_17
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6840-4_17
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6838-1
Online ISBN: 978-1-4939-6840-4
eBook Packages: Springer Protocols