Quantification of Cytokinin Levels and Responses in Abiotic Stresses

  • Alfonso AlbaceteEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)


Since their discovery in the 1950s, it has been established that cytokinins (CKs) play important regulatory roles in various physiological processes in plants. Only recently have CKs been also implicated in the response of plants to biotic and abiotic stresses. During the last years, several analytical methods have been developed to determine CK concentrations in plant tissues. Here we present a simple and robust method for CK extraction, purification and analysis in plant tissues, using ultrahigh-performance liquid chromatography coupled to high resolution mass spectrometry (U-HPLC-HRMS). The main advantage of this methodology is the simplicity of the purification protocol and the possibility to extend it to the analysis of other plant hormones and derivatives.

Key words

Plant hormones Cytokinins Abiotic stress Quantification Internal standards High-resolution mass spectrometry 



I would like to thank CSIC (Spain) for a postdoc research grant (JAE program), Prof. Francisco Pérez-Alfocea for supporting my research, and Ms. Cristina Soriano-Carpena for her help in editing the English version of the manuscript. Research funded by the Spanish MINECO-FEDER (project AGL2014-59197-JIN).


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Plant NutritionCEBAS-CSICMurciaSpain

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