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Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK)

  • Pallavi Singh
  • Alok Krishna SinhaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Plant physiology, in particular, is governed by a repertoire of endogenous as well as environmental cues. Auxin and cytokinin constitute an indispensable phytohormonal system required for plant growth and development. Another pivotal aspect of plant physiological process that thoroughly affects various plant growth and developmental attributes is the signaling network, majorly comprising the canonical mitogen activated protein kinase (MAPK) cascade. Striking a fine balance between the phytohormonal and signaling components could be adopted as an intricate strategy by plants to counteract various stresses in question. Thus, a brief understanding of this multifaceted complex could be of use for delineating numerous plant physiological and developmental phenomena. Thus, the present section discusses the various MAPK related assays in context to auxin and cytokinin crosstalk. Briefly, this chapter outlines the discrete MAPK methods to better understand the fundamentals of MAPK signaling network in auxin and cytokinin treated rice seedlings. Further, various phenotypic, genomic as well as proteomic protocols are discussed for a better understanding of MAPK networks in the backdrop of auxin and cytokinin interplay.

Key words

Auxin Cytokinin Mitogen activated protein kinase (MAPK) MAPK assays In-gel kinase assay Immunoblotting qRT-PCR Root structure architecture (RSA) 

Notes

Acknowledgments

The authors extend their gratitude to the Department of Biotechnology (DBT), Government of India and National Institute of Plant Genome Research (NIPGR), New Delhi, India for financial support. Council of Scientific and Industrial Research (CSIR), Government of India is duly acknowledged for providing fellowship to PS.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.National Institute of Plant Genome ResearchNew DelhiIndia

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