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Microbial Manipulation of Auxins and Cytokinins in Plants

  • Anwar HussainEmail author
  • Ihsan Ullah
  • Shahida Hasnain
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Microbial associations with plants are crucial for the survival of both the partners. Beside other ways of establishing such associations, phytohormones enjoy a key role in plant–microbe interactions from initial dialog between the two to the establishment of a viable partnership. Cytokinins (CKs) and IAA are among the five classical groups of phytohormones implicated in plant immune response, early signaling, and deciding the fate of interactions between plant and microbes. Here we describe a method to study modulation of Cks and IAA in plant under the influence of a pathogenic bacterium, Pseudomonas syringae tomato DC3000. A method for inoculating bacteria on host plant and subsequent determination of Cks and IAA through HPLC-ESI-MS/MS is described.

Key words

Cytokinin Auxins Plant immunity Pseudomonas syringae Arabidopsis Plant–microbe interactions 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of BotanyGarden Campus, Abdul Wali Khan UniversityMardanPakistan
  2. 2.Department of Environmental ScienceIslamic International UniversityIslamabadPakistan
  3. 3.Department of Microbiology and Molecular GeneticsUniversity of the PunjabLahorePakistan

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