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Analytical Determination of Auxins and Cytokinins

  • Petre I. DobrevEmail author
  • Klára Hoyerová
  • Jan Petrášek
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.

Key words

Auxin Cytokinins Phytohormones Liquid chromatography Mass spectrometry 

Notes

Acknowledgment

This work was supported by MEYS CR, projects no. LD14120 (PD), LD15137 (KH), and LO1417 (JP).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Petre I. Dobrev
    • 1
    Email author
  • Klára Hoyerová
    • 1
  • Jan Petrášek
    • 1
    • 2
  1. 1.Laboratory of Hormonal Regulations in Plants, Institute of Experimental BotanyCASPrague 6Czech Republic
  2. 2.Department of Experimental Plant Biology, Faculty of ScienceCharles UniversityPrague 2Czech Republic

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