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High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation

  • Nora Wehner
  • Jörn Herfert
  • Wolfgang Dröge-LaserEmail author
  • Christoph Weiste
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Genome sequencing and annotation studies clearly highlight the impact of transcriptional regulation in plants. However, functional characterization of the majority of transcriptional regulators remains elusive. Hence, high-throughput techniques are required to facilitate their molecular analysis. Here, we provide a detailed protocol to conduct a high-throughput protoplast trans-activation (PTA) screening, which enables simultaneous analysis of up to 95 individual transcription factor activities on a customizable promoter:LUCIFERASE reporter. This system is well suited to decipher complex transcriptional networks such as that triggered by the phytohormone auxin.

Key words

Transcription factor Screening High throughput Protoplast Gene regulation Auxin 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Nora Wehner
    • 1
  • Jörn Herfert
    • 1
  • Wolfgang Dröge-Laser
    • 1
    Email author
  • Christoph Weiste
    • 1
  1. 1.Pharmazeutische Biologie, Julius-von-Sachs-InstitutJulius-Maximilians-Universität WürzburgWürzburgGermany

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