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Analyzing Cytokinin Responses During Plant-Nematode Interactions

  • Florian M. W. Grundler
  • Shahid SiddiqueEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.

Key words

Cytokinins Nematode Giant cells Syncytium Cell cycle TCSn:GFP ARR5:GUS 

Notes

Acknowledgment

We are grateful to Zoran Radakovic, Julia Holbein, Christiane Matera, and Thomas Gerhardt for helping in various steps of protocol.

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Molecular PhytomedicineRheinische Friedrich-Wilhelms-University of BonnBonnGermany

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