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Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface

  • Muhammad NaseemEmail author
  • Shabana Shams
  • Thomas Roitsch
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1569)

Abstract

Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.

Key words

Cytokinins Pathogen-inducible promoter Host-pathogen interaction Transient plant transformation 

Notes

Acknowledgments

We are thankful to Anwar Hussain and Markus Kirschke for their help in analyzing tobacco samples for cytokinin determination. We appreciate DAAD (Deutscher Akademischer Austauschdienst) for providing doctoral fellowship to MN. This work was supported by funding from the Czech Ministry of Education, Youth and Sports of CR within the National Sustainability Program I (NPU I), grant number LO1415, to T.R.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Muhammad Naseem
    • 1
    • 2
    Email author
  • Shabana Shams
    • 3
  • Thomas Roitsch
    • 4
    • 5
  1. 1.Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg GermanyJulius-Maximilians-Universität WürzburgWürzburgGermany
  2. 2.Department of Molecular Biology and GeneticsBoğaziçi UniversityBebekTurkey
  3. 3.Department of Animal SciencesQuaid-i-Azam UniversityIslamabadPakistan
  4. 4.Department of Plant and Environmental Sciences, Copenhagen Plant Science CentreUniversity of CopenhagenTaastrupDenmark
  5. 5.Global Change Research CentreCzech Globe AS CRDrásovCzech Republic

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