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Cryopreservation of Mammalian Gametes and Embryos pp 85–104Cite as

Chapter 7 Human Epididymal and Testicular Sperm Cryopreservation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

Since the advent of intracytoplasmic sperm injection (ICSI) in the early 1990s, surgical techniques to recover samples from the epididymis and testis directly have been used to benefit patients suffering from obstructive and nonobstructive azoospermia. Various studies have demonstrated comparable fertilization, ongoing pregnancy, and implantation rates when fresh and frozen-thawed epididymal sperms were used for ICSI [1]. Injection of fresh and frozen testicular sperms into mature oocytes resulted in similar fertilization rates in cases of obstructive azoospermia. However, in cases of nonobstructive azoospermia, the outcome depends upon the degree of impairment of spermatogenesis, criteria for sperm freezing, and patient selection [2].

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Author information

Authors and Affiliations

  1. ART Centre, Army Hospital (Research and Referral), Dhaula Kuan, New Delhi, 110010, India

    Pankaj Talwar

  2. Reproductive Medicine, Artemis Health Institute, Sector-51, Gurgaon, Haryana, 122011, India

    Sarabpreet Singh

Authors
  1. Pankaj Talwar
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  2. Sarabpreet Singh
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Corresponding author

Correspondence to Pankaj Talwar .

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Talwar, P., Singh, S. (2017). Chapter 7 Human Epididymal and Testicular Sperm Cryopreservation. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

  • eBook Packages: Springer Protocols

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