Chapter 16 Directional Freezing of Ovarian Tissue and Freeze-Drying of Stem Cells for Fertility Preservation

  • Amir AravEmail author
  • Yehudit Natan
Part of the Methods in Molecular Biology book series (MIMB, volume 1568)


Fertility preservation is practiced today for women in their fertile years who wish or need to postpone or prolong their reproductive era. Currently women have two main options to preserve their fertility either by oocyte vitrification or by freezing of ovarian cortical slices. In this chapter we will describe the use of directional freezing for cortical slices and freeze-drying of stem cells. These procedures open the way for the preservation of reproductive cells and tissue for future use in fertility preservation treatments.

Key words

Fertility preservation Cryopreservation Freezing Directional freezing Freeze-drying Ovary Stem cells Cortical slices 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Fertilesafe Ltd.Ness ZionaIsrael

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