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Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

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Thermogenic Fat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1566))

Abstract

Human brown and white preadipocytes offer unique cell models to study human adipogenesis and thermogenesis. Here, we describe the detailed procedures for isolation of human brown and white predipocytes from deep and superficial neck fat. To grow these cells in vitro for a prolonged period of time, they should be immortalized following the procedure discussed here. We also provide the protocol for expansion, cryopreservation, and adipogenic differentiation of cells.

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Acknowledgments

This work was supported in part by NIH grants R01 DK077097 and R01 DK102898 (to Y.-H.T.), and P30 DK036836 (to Joslin Diabetes Research Center).

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Correspondence to Yu-Hua Tseng .

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Shamsi, F., Tseng, YH. (2017). Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6820-6_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6819-0

  • Online ISBN: 978-1-4939-6820-6

  • eBook Packages: Springer Protocols

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