Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

  • Longhua Liu
  • Louise D. Zheng
  • Sarah R. Donnelly
  • Margo P. Emont
  • Jun Wu
  • Zhiyong Cheng
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed.

Key words

Adipose tissue Stromal vascular fraction Adipogenesis Lipid accumulation Energy balance Obesity 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Longhua Liu
    • 1
  • Louise D. Zheng
    • 1
  • Sarah R. Donnelly
    • 1
  • Margo P. Emont
    • 2
  • Jun Wu
    • 2
  • Zhiyong Cheng
    • 1
  1. 1.Department of Human Nutrition, Foods and ExerciseFralin Life Science Institute, College of Agriculture and Life Science, Virginia TechBlacksburgUSA
  2. 2.Department of Molecular and Integrative PhysiologyLife Sciences Institute, University of MichiganAnn ArborUSA

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