Designing 3-D Adipospheres for Quantitative Metabolic Study

  • Takeshi Akama
  • Brendan M. Leung
  • Joe Labuz
  • Shuichi Takayama
  • Tae-Hwa Chun
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Quantitative assessment of adipose mitochondrial activity is critical for better understanding of adipose tissue function in obesity and diabetes. While the two-dimensional (2-D) tissue culture method has been sufficient to discover key molecules that regulate adipocyte differentiation and function, the method is insufficient to determine the role of extracellular matrix (ECM) molecules and their modifiers, such as matrix metalloproteinases (MMPs), in regulating adipocyte function in three-dimensional (3-D) in vivo-like microenvironments. By using a 3-D hanging drop tissue culture system, we are able to produce scalable 3-D adipospheres that are suitable for quantitative metabolic study in 3-D microenvironment.

Key words

Spheroid Drop culture Adipocyte Mitochondria 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Takeshi Akama
    • 1
    • 2
  • Brendan M. Leung
    • 2
    • 3
  • Joe Labuz
    • 2
    • 3
  • Shuichi Takayama
    • 2
    • 3
  • Tae-Hwa Chun
    • 1
    • 2
  1. 1.Division of Metabolism, Endocrinology & Diabetes, Department of Internal Medicine,University of Michigan Medical SchoolAnn ArborUSA
  2. 2.Biointerfaces InstituteUniversity of MichiganAnn ArborUSA
  3. 3.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA

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