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Identification and Quantification of Human Brown Adipose Tissue

  • Maria Chondronikola
  • Craig Porter
  • John O. Ogunbileje
  • Labros S. Sidossis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Brown adipose tissue (BAT) has attracted significant interest as a potential target tissue against obesity and its associated metabolic perturbations. The purpose of this chapter is to provide an overview of some of the methodological approaches that can be used to identify and quantify BAT in people. Specifically, we will provide a step-by-step description of the following procedures: quantification of BAT in vivo using positron emission tomography-computed tomography (PET/CT) with 2-deoxy-2-[18F]fluoroglucose (18F-FDG) as a tracer, mitochondrial respiration, and uncoupling protein 1 (UCP1) gene and protein expression.

Key words

Brown adipose tissue Positron emission tomography-computed tomography Uncoupling protein 1 2-deoxy-2-[18F]fluoroglucose Biopsy 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Maria Chondronikola
    • 1
    • 2
  • Craig Porter
    • 3
    • 4
  • John O. Ogunbileje
    • 3
    • 4
  • Labros S. Sidossis
    • 2
    • 3
    • 4
    • 5
    • 6
  1. 1.Center for Human Nutrition, School of MedicineWashington University in St. LouisSt. LouisUSA
  2. 2.Harokopio University of AthensAthensGreece
  3. 3.Department of SurgeryUniversity of Texas Medical Branch at GalvestonGalvestonUSA
  4. 4.Metabolism Unit, Shriners Hospitals for Children-GalvestonGalvestonUSA
  5. 5.Department of MedicineRutgers UniversityNew BrunswickUSA
  6. 6.Department of Kinesiology and HealthRutgers UniversityNew BrunswickUSA

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