Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry
Global obesity epidemic demands more effective therapeutic treatments and better understanding of obesity pathophysiology. Since obesity results from energy imbalance, accurate quantification of energy intake and energy expenditure (EE) becomes an essential prerequisite to phenotype the cause for obesity development. Indirect calorimetry has long been used as one of the most established methods in EE quantification by detecting changes in levels of O2 consumption and CO2 production. In this article, we describe procedures and important considerations for an effective measurement using indirect calorimetry.
Key wordsIndirect calorimetry Energy expenditure Metabolic rate Oxygen consumption Obesity
This work was supported by NIH R01DK092605. Q.T. is the holder of Cullen Chair in Molecular Medicine and Welch Research Scholar (L-AU0002) of the University of Texas McGovern Medical School.
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