Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Global obesity epidemic demands more effective therapeutic treatments and better understanding of obesity pathophysiology. Since obesity results from energy imbalance, accurate quantification of energy intake and energy expenditure (EE) becomes an essential prerequisite to phenotype the cause for obesity development. Indirect calorimetry has long been used as one of the most established methods in EE quantification by detecting changes in levels of O2 consumption and CO2 production. In this article, we describe procedures and important considerations for an effective measurement using indirect calorimetry.

Key words

Indirect calorimetry Energy expenditure Metabolic rate Oxygen consumption Obesity 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Brown Foundation Institute of Molecular MedicineUniversity of Texas McGovern Medical SchoolHoustonUSA
  2. 2.Department of Neurobiology and AnatomyUniversity of Texas McGovern Medical SchoolHoustonUSA

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