Bioenergetic Analyses in Adipose Tissue

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Adipose tissue plays an important role in whole body metabolism, and its dysfunction results in poor metabolic health and is associated with a diverse set of disorders, such as obesity, cardiovascular disease, hypertension, cancer, and type 2 diabetes. The search for cellular processes that can be targeted to activate adipose oxidative metabolism is thought to hold promise for combating these diseases, and so the analysis of adipose bioenergetics is crucial toward this end. A description is provided here for the preparation of mitochondria and primary adipocytes from adipose tissue to be used for bioenergetics profiling.

Key words

Mitochondria Brown fat Beige fat Bioenergetics Primary adipocytes Stromal vascular fraction 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Dana-Faber Cancer Institute, Harvard Medical School, Cancer Biology CLS 111146BostonUSA

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