Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Abstract

Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.

Key words

Adipose tissue Brown fat White fat Beige fat Adipocyte Viral vectors Adenovirus Transduction Retrovirus Lentivirus Adeno-associated virus AAV Adipogenesis Adipokine Metabolism Diabetes Obesity 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Metabolic Health Center, Division of Cardiology, Department of MedicineWeill Cornell MedicineNew YorkUSA

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