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In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina

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Morpholino Oligomers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1565))

Abstract

Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.

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Acknowledgements

This work was supported by P30 from National Institute of Neurological Disorders and Stroke (5P30NS069266) and K99-R00 from National Eye Institute (4R00EY019547) to R.K. We would like to thank Edward J. Lechowicz, Jr. at the University of Connecticut for building the explant electroporation chamber.

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Correspondence to Rahul Kanadia .

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Karunakaran, D.K.P., Kanadia, R. (2017). In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina. In: Moulton, H., Moulton, J. (eds) Morpholino Oligomers. Methods in Molecular Biology, vol 1565. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6817-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6817-6_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6815-2

  • Online ISBN: 978-1-4939-6817-6

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