Abstract
Morpholino oligomers (MOs) are antisense molecules designed for sequence-specific binding of target mRNA. In bacteria, inhibition is hypothesized to occur by preventing translation initiation. Cell-penetrating peptides may be conjugated to the 5′- or 3′-termini of an MO to enhance cellular entry and therefore inhibition. Here we describe the three standard microbiological assays to assess in vitro antibacterial MO efficacy.
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Daly, S.M., Sturge, C.R., Greenberg, D.E. (2017). Inhibition of Bacterial Growth by Peptide-Conjugated Morpholino Oligomers. In: Moulton, H., Moulton, J. (eds) Morpholino Oligomers. Methods in Molecular Biology, vol 1565. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6817-6_10
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DOI: https://doi.org/10.1007/978-1-4939-6817-6_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6815-2
Online ISBN: 978-1-4939-6817-6
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