Brassinosteroids pp 23-29 | Cite as
Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
- 5 Citations
- 1.7k Downloads
Abstract
Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with BR responses. Here, we describe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (Oryza sativa) seedlings.
Key words
Brassinosteroid sensitivity Brassinosteroid response Lamina inclination Coleoptile elongation RiceNotes
Acknowledgments
This work was supported by grants from National Natural Science Foundation of China (91435106, 91335203, and 31170715) and Youth Innovation Promotion Association, Chinese Academy of Sciences (2015076 to H.T.).
References
- 1.Wada K, Marumo S, Abe H et al (1984) A rice lamina inclination test—a micro-quantitative bioassay for brassinosteroids. Agric Biol Chem 48:719–726Google Scholar
- 2.Fujioka S, Noguchi T, Takatsuto S et al (1998) Activity of brassinosteroids in the dwarf rice lamina inclination bioassay. Phytochemistry 49:1841–1848CrossRefGoogle Scholar
- 3.Hong Z, Ueguchi-Tanaka M, Umemura K et al (2003) A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell 15:2900–2910CrossRefPubMedPubMedCentralGoogle Scholar
- 4.Tong H, Xiao Y, Liu D et al (2014) Brassinosteroid regulates cell elongation by modulating gibberellin metabolism in rice. Plant Cell 26:4376–4393CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Sun S, Chen D, Li X et al (2015) Brassinosteroid signaling regulates leaf erectness in Oryza sativa via the control of a specific U-type cyclin and cell proliferation. Dev Cell 34:220–228CrossRefPubMedGoogle Scholar
- 6.Tong H, Liu L, Jin Y et al (2012) DWARF AND LOW-TILLERING acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice. Plant Cell 24:2562–2577CrossRefPubMedPubMedCentralGoogle Scholar