Abstract
Plasma membrane-localized receptors are essential for cellular communication and signal transduction. In Arabidopsis thaliana, BRASSINOSTEROID INSENSITIVE1 (BRI1) is one of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endomembrane trafficking. Advances in fluorescence labeling and confocal microscopy techniques enabled us to gain a better understanding of plasma membrane receptor dynamics in living cells. Here we describe different quantitative microscopy methods to monitor the relative steady-state levels of the BRI1 protein in the plasma membrane of root epidermal cells and its relative exocytosis and recycling rates. The methods can be applied also to analyze similar dynamics of other plasma membrane-localized receptors.
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Acknowledgements
We thank our colleagues Daniel Van Damme for help with the image analysis and critical reading of the manuscript, Evelien Mylle for technical assistance with the confocal microscope, and Martine De Cock for help in preparing the manuscript. Y.L. is indebted to the Belgian Science Policy Office (BELSPO) for a postdoctoral fellowship.
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Luo, Y., Russinova, E. (2017). Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells. In: Russinova, E., Caño-Delgado, A. (eds) Brassinosteroids. Methods in Molecular Biology, vol 1564. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6813-8_10
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DOI: https://doi.org/10.1007/978-1-4939-6813-8_10
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