Abstract
The unicellular green alga, Penium margaritaceum, represents a novel and valuable model organism for elucidating cell wall dynamics in plants. This organism’s cell wall contains several polymers that are highly similar to those found in the primary cell walls of land plants. Penium is easily grown in laboratory culture and is effectively manipulated in various experimental protocols including microplate assays and correlative microscopy. Most importantly, Penium can be live labeled with cell wall-specific antibodies or other probes and returned to culture where specific cell wall developmental events can be monitored. Additionally, live cells can be rapidly cryo-fixed and cell wall surface microarchitecture can be observed with variable pressure scanning electron microscopy. Here, we describe the methodology for maintaining Penium for experimental cell wall enzyme studies.
This is a preview of subscription content, log in via an institution to check access.
References
Oikawa A, Lund CH, Sakuragi Y, Scheller HV (2013) Golgi-localized enzyme complexes for plant cell wall biosynthesis. Trends Plant Sci 18:49–58
Li S, Lei L, Yingling YG, Gu Y (2015) Microtubules and cellulose biosynthesis: the emergence of new players. Curr Opin Plant Biol 28:76–82
Anderson CT (2016) We be jammin’: an update on pectin biosynthesis, trafficking and dynamics. J Exp Bot 67:495–502
Cosgrove DJ (2016) Plant cell wall extensibility: connecting plant cell growth with cell wall structure, mechanics, and the action of wall-modifying enzymes. J Exp Bot 67:463–476
Keegstra K (2010) Plant cell walls. Plant Physiol 154:483–486
Doblin MS, Pettolino F, Bacic A (2010) Plant cell walls: the skeleton of the plant world. Funct Plant Biol 37:357–381
Cosgrove DJ, Jarvis MC (2012) Comparative structure and biomechanics of plant primary and secondary cell walls. Front Plant Sci3:204.http://www.frontiersin.org/Plant_Physiology/10.3389/fpls.2012.00204/full
Domozych DS, Sorensen I, Popper ZA, Ochs J, Andreas A, Fangel JU, Pielach A, Sachs C, Brechka H, Ruisi-Besares P, Willats WGT, Rose JKC (2014) Pectin metabolism and assembly in the cell wall of the charophyte green alga Penium margaritaceum. Plant Physiol 165:105–118
Domozych DS, Sørensen I, Sacks C et al (2014) Disruption of the microtubule network alters cellulose deposition and causes major changes in pectin distribution in the cell wall of the green algaPenium margaritaceum. J Exp Bot 65:465–479
Domozych DS, Lambiasse L, Kiemle SN, Gretz MR (2009) Cell-wall development and bipolar growth in the desmid Penium margaritaceum (Zygnematophyceae, Streptophyta). Asymmetry in a symmetric world. J Phycol 45:879–893
Gilbert HJ, Knox JP, Boraston AB (2013) Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules. Curr Opin Struct Biol 23:669–677
Mravec JJ, Kračun SK, Rydahl MG, Westereng B, Miart F, Clausen MH, Fangel JU, Daugaard M, Van Cutsem P, De Fine LHH, Höfte H, Malinovsky FG, Domozych DS, Willats WGT (2014) Tracking developmentally regulated post-synthetic processing of homogalacturonan and chitin using reciprocal oligosaccharide probes. Development 141:4841–4850
Acknowledgments
This work was supported by NSF grant NSF-MCB-RUI-1517345.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Domozych, D., Lietz, A., Patten, M., Singer, E., Tinaz, B., Raimundo, S.C. (2017). Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum . In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6810-7_7
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6808-4
Online ISBN: 978-1-4939-6810-7
eBook Packages: Springer Protocols