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Introduction to Modern Methods in Light Microscopy

Part of the Methods in Molecular Biology book series (MIMB,volume 1563)

Abstract

For centuries, light microscopy has been a key method in biological research, from the early work of Robert Hooke describing biological organisms as cells, to the latest in live-cell and single-molecule systems. Here, we introduce some of the key concepts related to the development and implementation of modern microscopy techniques. We briefly discuss the basics of optics in the microscope, super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.

Key words

  • Microscopy
  • Technology
  • Super-resolution
  • Image analysis
  • Live-cell

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Correspondence to Paul S. Maddox .

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Ryan, J., Gerhold, A.R., Boudreau, V., Smith, L., Maddox, P.S. (2017). Introduction to Modern Methods in Light Microscopy. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_1

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