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Automated Analysis of Intracellular Dynamic Processes

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Light Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

The study of intracellular dynamic processes is of fundamental importance for understanding a wide variety of diseases and developing effective drugs and therapies. Advanced fluorescence microscopy imaging systems nowadays allow the recording of virtually any type of process in space and time with super-resolved detail and with high sensitivity and specificity. The large volume and high information content of the resulting image data, and the desire to obtain objective, quantitative descriptions and biophysical models of the processes of interest, require a high level of automation in data analysis. Two key tasks in extracting biologically meaningful information about intracellular dynamics from image data are particle tracking and particle trajectory analysis. Here we present state-of-the-art software tools for these tasks and describe how to use them.

*These authors contributed equally to this work

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Acknowledgments

This work was supported by the Dutch Technology Foundation (STW Grants 10443 and 13391).

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Author notes

    Authors and Affiliations

    1. Departments of Medical Informatics and Radiology, Biomedical Imaging Group Rotterdam, Erasmus University Medical Center, 2040, 3000 CA, Rotterdam, The Netherlands

      Yao Yao, Ihor Smal & Erik Meijering

    2. Department of Cell Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

      Ilya Grigoriev, Maud Martin & Anna Akhmanova

    Authors
    1. Yao Yao
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    2. Ihor Smal
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    3. Ilya Grigoriev
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    4. Maud Martin
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    5. Anna Akhmanova
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    6. Erik Meijering
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    Corresponding author

    Correspondence to Erik Meijering .

    Editor information

    Editors and Affiliations

    1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

      Yolanda Markaki

    2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

      Hartmann Harz

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    Yao, Y., Smal, I., Grigoriev, I., Martin, M., Akhmanova, A., Meijering, E. (2017). Automated Analysis of Intracellular Dynamic Processes. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_14

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    • DOI: https://doi.org/10.1007/978-1-4939-6810-7_14

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6808-4

    • Online ISBN: 978-1-4939-6810-7

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