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Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation

  • Bi-Feng YuanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1562)

Abstract

Dynamic RNA modifications recently were considered to constitute another realm for biological regulation in the form of “RNA epigenetics”. N6-methyladenosine (m6A), one of the most important modifications on RNA, plays a fundamental role in epigenetic regulation of the mammalian transcriptome. We recently established various liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)-based methods for the sensitive and accurate determination of modified nucleosides in both DNA and RNA. Here, we describe a protocol to analyze m6A in RNA by LC-ESI-MS/MS. And this protocol also can be extended to the analysis of other modified nucleosides in both DNA and RNA.

Key words

RNA Methylation, N6-Methyladenosine; Liquid Chromatography-Mass Spectrometry 

Notes

Acknowledgments

The author thanks the financial support from the National Natural Science Foundation of China (21522507, 21672166).

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of ChemistryWuhan UniversityWuhanChina

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