Methylated mRNA Nucleotides as Regulators for Ribosomal Translation

  • Thomas P. Hoernes
  • Matthias D. ErlacherEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1562)


Methylated RNA nucleotides were recently discovered to be highly abundant in RNAs. The effects of these methylations were mainly attributed to altered mRNA stabilities, protein-binding affinities, or RNA structures. The direct impact of RNA modifications on the performance of the ribosome has not been investigated so far. In this chapter, we describe an approach that allows introducing RNA modifications site-specifically into coding sequences of mRNAs and determining their effect on the translation machinery in a well-defined bacterial in vitro system.

Key words

Splinted ligation In vitro translation RNA purification Peptide purification 



This work was supported by the FWF (P 22658-B12 and P 28494-BBL to M.E.)


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Division of Genomics and RNomics, Biocenter InnsbruckMedical University of InnsbruckInnsbruckAustria

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