Transcriptome-Wide Mapping of N1-Methyladenosine Methylome

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1562)

Abstract

N1-Methyladenosine (m1A) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of m1A in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of m1A will be important for further functional investigations. Here, we describe a technique called “m1A-ID-Seq”, which is based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, to map m1A in the transcriptome. Utilizing this technique, highly confident m1A peaks can be obtained on a transcriptome-wide level.

Key words

N1-Methyladenosine (m1A) Transcriptome-wide profiling AlkB Demethylase m1A-ID-Seq Epitranscriptome RNA epigenetics 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Protein and Plant Gene ResearchSchool of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking UniversityBeijingChina
  2. 2.Department of Chemical Biology, Synthetic and Functional Biomolecules CenterCollege of Chemistry and Molecular Engineering, Peking UniversityBeijingChina

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