Analysis of High-Throughput RNA Bisulfite Sequencing Data

  • Dietmar RiederEmail author
  • Francesca Finotello
Part of the Methods in Molecular Biology book series (MIMB, volume 1562)


Methylation of the 5-cytosine (m5C) is a common but not well-understood RNA modification, which can be detected by sequencing of bisulfite-treated transcripts (RNA-BSseq). In this Chapter, we discuss computational RNA-BSseq data analysis methods for transcriptome-wide identification and quantification of m5C.

Key words

RNA-BSseq RNA methylation m5Differential methylation 5-methylcytosine Transcription RNA modification Bisulfite conversion High-throughput sequencing Data analysis RNA Modification (Cytosine-5) methylation Sodium bisulfite 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Division of Bioinformatics, BiocenterMedical University of InnsbruckInnsbruckAustria

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