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Histochemical Demonstration of Tripeptidyl Aminopeptidase I

  • Mashenka B. Dimitrova
  • Dimitrinka Y. Atanasova
  • Nikolai E. LazarovEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI—glycyl-l-prolyl-l-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-l-prolyl-l-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs.

Key words

Enzyme histochemistry Chromogenic and fluorogenic substrates Brightfield microscopy Fluorescence microscopy Mouse Rat Tripeptidyl peptidase I 

Notes

Acknowledgments

The authors greatly thank Prof Dr. Michail Davidoff for protocol consultation.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Mashenka B. Dimitrova
    • 1
  • Dimitrinka Y. Atanasova
    • 2
    • 3
  • Nikolai E. Lazarov
    • 2
    • 4
    Email author
  1. 1.Institute of Experimental Morphology, Pathology and Anthropology with MuseumBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of NeurobiologyBulgarian Academy of SciencesSofiaBulgaria
  3. 3.Department of Anatomy, Faculty of MedicineTrakia UniversityStara ZagoraBulgaria
  4. 4.Department of Anatomy and HistologyMedical University of SofiaSofiaBulgaria

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