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Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

  • Vittorio BertoneEmail author
  • Eleonora Tarantola
  • Isabel Freitas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

Key words

Dipeptidyl-peptidase IV Enzyme histochemistry In situ enzyme activity Cryostatic samples 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Vittorio Bertone
    • 1
    Email author
  • Eleonora Tarantola
    • 1
  • Isabel Freitas
    • 1
  1. 1.Department of Biology and Biotechnology “Lazzaro Spallanzani”University of PaviaPaviaItaly

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